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JoVE Online Video Presentations
Journal of Visualized Experiments (JoVE) is a peer reviewed, PubMed indexed journal devoted to the publication of biological, medical, chemical and physical research in a video format.
The video-articles listed below use PerkinElmer in vivo imaging systems and/or imaging reagents. Video-articles are either open access, in which you can view the full video or a subscription is required to view the video in its entirity.
Open Access Videos
| Combined In vivo Optical and μCT Imaging to Monitor Infection, Inflammation, and Bone Anatomy in an Orthopaedic Implant Infection in Mice Nicholas M. Bernthal1, Brad N. Taylor2, Jeffrey A. Meganck2, Yu Wang3, Jonathan H. Shahbazian3, Jared A. Niska1, Kevin P. Francis2, Lloyd S. Miller3,4 1Orthopaedic Hospital Research Center, Orthopaedic Hospital Department of Orthopaedic Surgery, David Geffen School of Medicine at University of California, Los Angeles (UCLA), 2PerkinElmer, 3Department of Dermatology, Johns Hopkins University School of Medicine, 4Department of Medicine, Division of Infectious Diseases, Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine Combined optical and μCT imaging in a mouse model of orthopaedic implant infection, utilizing a bioluminescent engineered strain of Staphylococcus aureus, provided the capability to noninvasively and longitudinally monitor the dynamics of the bacterial infection, as well as the corresponding inflammatory response and anatomical changes in the bone. PRODUCTS: IVIS Spectrum, Quantum FX, Living Image software, Xen29 bioluminescent Staphylococcus aureus strain |
| Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue Xueli Zhang1,2, Chaincy Kuo3, Anna Moore1, Chongzhao Ran1 1Molecular Imaging Laboratory, MGH/MIT/HMS Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital/Harvard Medical School, 2Center for Drug Discovery, School of Pharmacy, China Pharmaceutical University, 3Perkin Elmer
In this video report, we show the application of Cerenkov Luminescence Imaging (CLI) for interscapular brown adipose tissue in mice under activated and depressed conditions.
PRODUCTS: IVIS Spectrum |
| 4D Multimodality Imaging of Citrobacter rodentium Infections in Mice James William Collins1, Jeffrey A Meganck2, Chaincy Kuo2, Kevin P Francis2, Gad Frankel1 1MRC Centre for Molecular Bacteriology and Infection, Division of Cell & Molecular Biology, Imperial College London, 2Preclinical Imaging, PerkinElmer
Multi-modality imaging is a valuable approach for studying bacterial colonization in small animal models. This protocol outlines infection of mice with bioluminescent Citrobacter rodentium and the longitudinal monitoring of bacterial colonization using composite 3D diffuse light imaging tomography with μCT imaging to create a 4D movie of C. rodentium infection. PRODUCTS: IVIS SpectrumCT, XGI-8 gas anesthesia system, Living Image 4.3.1 software |
| In Vivo Imaging Systems (IVIS) Detection of a Neuro-Invasive Encephalitic Virus Allison Poussard*, Michael Patterson*, Katherine Taylor, Alexey Seregin, Jeanon Smith, Jennifer Smith, Milagros Salazar, Slobodan Paessler
Experimental Pathology, University of Texas Medical Branch
Utilizing luciferase and in vivo imaging systems (IVIS) as a novel means to identify disease endpoints before clinical developments occur. IVIS has allowed us to visualize in real time the invasion of encephalitic viruses over multiple days, providing a more accurate disease model for future study. It has also allowed us to identify the potential protective features of antivirals and vaccines faster than currently utilized animal models. The capability to utilize individual animals over multiple time points ensures reduced animal requirements, costs, and overall morbidity to the animals utilized ensuring a more humane and more scientific means of disease study. PRODUCTS: IVIS Spectrum, XGI-8 gas anesthesia system, Living Image 4.0 software |
| Longitudinal Evaluation of Mouse Hind Limb Bone Loss After Spinal Cord Injury using Novel, in vivo, Methodology Madonna M. McManus, Raymond J. Grill
Department of Integrative Biology and Pharmacology, University of Texas Health Science Center at Houston
A longitudinal examination of bone loss in the femurs and tibiae of adult mice was performed following spinal cord injury using sequential low-dose X-ray scans. Tibia bone loss was detected throughout the study, while bone loss in the femur was not detected until 40 days post injury. PRODUCTS: IVIS Lumina XR |
| Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy Peter Bannas*1, Alexander Lenz*1,2, Valentin Kunick1,2, William Fumey1,2, Bjorn Rissiek2, Joanna Schmid1,2, Friedrich Haag2, Axel Leingartner3, Martin Trepel4, Gerhard Adam1, Friedrich Koch-Nolte2 1Department of Diagnostic and Interventional Radiology, University Medical Center, Hamburg, 2Institute of Immunology, University Medical Center, Hamburg, 3University Cancer Center Hamburg, University Medical Center, Hamburg, 4Department of Oncology and Hematology, University Medical Center, Hamburg
This protocol outlines the steps required to perform ex vivo validation of in vivo near-infrared fluorescence xenograft imaging experiments in mice using fluorophore labelled nanobodies and conventional antibodies. PRODUCTS: IVIS 100 | | Systemic and Local Drug Delivery for Treating Diseases of the Central Nervous System in Rodent Models PRODUCTS: IVIS Lumina | | Intracranial Implantation with Subsequent 3D In Vivo Bioluminescent Imaging of Murine Gliomas PRODUCTS: IVIS Spectrum, Luciferin | | In vivo Dual Substrate Bioluminescent Imaging PRODUCTS: IVIS 200, RediJect Coelenterazine, D-Luciferin | | Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems PRODUCTS: IVIS Lumina II | | Ex Vivo Infection of Live Tissue with Oncolytic Viruses PRODUCTS: IVIS 200 | | Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice PRODUCTS: IVIS Spectrum | | Monitoring Tumor Metastases and Osteolytic Lesions with Bioluminescence and Micro CT Imaging PRODUCTS: IVIS Spectrum, Quantum FX uCT, Light Producing Cells (Bioware) | | In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer PRODUCTS: IVIS Spectrum, D-Ludiferin | | Establishing Intracranial Brain Tumor Xenografts With Subsequent Analysis of Tumor Growth and Response to Therapy using Bioluminescence Imaging PRODUCTS: IVIS Lumina | | In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum PRODUCTS: IVIS Spectrum | | Establishing Intracranial Brain Tumor Xenografts With Subsequent Analysis of Tumor Growth and Response to Therapy using Bioluminescence Imaging PRODUCTS: IVIS Lumina | | In vivo Bioluminescent Imaging of Mammary Tumors Using IVIS Spectrum PRODUCTS: IVIS Spectrum | | Use of Human Perivascular Stem Cells for Bone Regeneration
PRODUCTS: IVIS Lumina II, Living Image | | Orthotopic Xenografting of Human Luciferase-Tagged Malignant Peripheral Nerve Sheath Tumor Cells for in vivo Testing of Candidate Therapeutic Agents
PRODUCTS: IVIS Imaging System, D-Luciferin |
A JoVE Subscription is Required To View These Videos in Their Entirity
| Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination Lukasz J. Ochyl1,2, James J Moon1,2,3 1Department of Pharmaceutical Sciences, University of Michigan, 2Biointerfaces Institute, University of Michigan, 3Department of Biomedical Engineering, University of Michigan
We describe whole-animal imaging and flow cytometry-based techniques for monitoring expansion of antigen-specific CD8+ T cells in response to immunization with nanoparticles in a murine model of vaccination.
PRODUCTS: IVIS Series |
| Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria Malek Saleh1, Mohammed R. Abdullah1, Christian Schulz1, Thomas Kohler1, Thomas Pribyl1, Inga Jensch1, Sven Hammerschmidt1 1Department Genetics of Microorganisms, Interfaculty Institute for Genetics and Functional Genomics, University of Greifswald
Streptococcus pneumoniae is the leading pathogen causing severe community-acquired pneumonia and responsible for over 2 million deaths worldwide. The impact of bacterial factors implicated in fitness or virulence can be monitored in real-time in an acute mouse pneumonia or bacteremia model using bioluminescent bacteria.
PRODUCTS: IVIS Spectrum, Living Image 4.1 Software, XGI-8 Gas Anesthesia System |
| In vivo Imaging Method to Distinguish Acute and Chronic Inflammation Jen-Chieh Tseng1, Andrew L. Kung2 1Lurie Family Imaging Center, Dana-Farber Cancer Institute, Harvard Medical School, 2Division of Pediatric Hematology/Oncology/Stem Cell Transplantation, Columbia University Medical Center
We describe a non-invasive imaging method for distinguishing inflammatory stages. Systemic delivery of luminol reveals areas of acute inflammation dependent upon MPO activity in neutrophils. In contrast, injection of lucigenin allows for visualization of chronic inflammation dependent upon Phox activity in macrophages.
PRODUCTS: IVIS Spectrum, Living Image 4.2 Software |
| Cerenkov Luminescence Imaging (CLI) for Cancer Therapy Monitoring Yingding Xu, Hongguang Liu, Edwin Chang, Han Jiang, Zhen Cheng
Department of Radiology and Bio-X Program Canary Cancer at Stanford for Cancer Early Detection, Stanford University
Use of Cerenkov Luminescence Imaging (CLI) for monitoring preclinical cancer treatment is described here. This method takes advantage of Cerenkov Radiation (CR) and optical imaging (OI) to visualize radiolabeled probes and thus provides an alternative to PET in preclinical therapeutic monitoring and drug screening. PRODUCTS: IVIS Spectrum | | Bioluminescent Bacterial Imaging In Vivo PRODUCTS: IVIS | | Stereotactic Intracranial Implantation and In vivo Bioluminescent Imaging of Tumor Xenografts in a Mouse Model System of Glioblastoma Multiforme PRODUCTS: IVIS Lumina | | Models of Bone Metastasis PRODUCTS: IVIS Imaging System | | Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic PRODUCTS: IVIS 200 | | Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model PRODUCTS: IVIS 200 | | Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice PRODUCTS: D-Luciferin | | An Orthotopic Bladder Tumor Model and the Evaluation of Intravesical saRNA Treatment PRODUCTS: IVIS Spectrum, Quantum FX uCT, Light Producing Cells (Bioware) | | A Cre-Lox P Recombination Approach for the Detection of Cell Fusion In Vivo PRODUCTS: IVIS Spectrum, D-Luciferin | | In vivo Bioluminescence Imaging of Tumor Hypoxia Dynamics of Breast Cancer Brain Metastasis in a Mouse Model PRODUCTS: IVIS Spectrum | | Using Luciferase to Image Bacterial Infections in Mice PRODUCTS: IVIS Imaging System | | An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses PRODUCTS: IVIS Lumina | | In vivo Imaging of Transgenic Leishmania Parasites in a Live Host PRODUCTS: IVIS 200, D-Luciferin | | Bioluminescence Imaging of Heme Oxygenase-1 Upregulation in the Gua Sha Procedure PRODUCTS: IVIS 200, D-Luciferin | | Embryonic Stem Cell-Derived Endothelial Cells for Treatment of Hindlimb Ischemia PRODUCTS: IVIS 200 | | In vitro and in vivo Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cells PRODUCTS: IVIS Imaging System | | DNA Vector-based RNA Interference to Study Gene Function in Cancer
PRODUCTS: IVIS Imaging System | | Murine Bioluminescent Hepatic Tumour Model
PRODUCTS: IVIS Imaging System | | Ex Vivo Culture of Patient Tissue & Examination of Gene Delivery
PRODUCTS: IVIS Imaging System |
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