In vivo Quantitative Assessment of Myocardial Structure, Function, Perfusion and Viability using MicroCT Imaging

Elza van Deel^1,2, Yanto Ridwan¹, J. Nicole van Vliet¹, Sasha Belenkov³, Jeroen Essers^1,4,5
1Department of Genetics, Erasmus MC, Rotterdam, ²Department of Experimental Cardiology, Erasmus MC, Rotterdam, ³PerkinElmer, Inc., ⁴Department of Vascular Surgery, Erasmus MC, Rotterdam, ⁵Department of Radiation Oncology, Erasmus MC, Rotterdam

Combined In vivo Optical and μCT Imaging to Monitor Infection, Inflammation, and Bone Anatomy in an Orthopaedic Implant Infection in Mice

Nicholas M. Bernthal¹, Brad N. Taylor², Jeffrey A. Meganck², Yu Wang³, Jonathan H. Shahbazian³, Jared A. Niska¹, Kevin P. Francis², Lloyd S. Miller^3,4
1Orthopaedic Hospital Research Center, Orthopaedic Hospital Department of Orthopaedic Surgery, David Geffen School of Medicine at University of California, Los Angeles (UCLA), ²PerkinElmer, ³Department of Dermatology, Johns Hopkins University School of Medicine, ⁴Department of Medicine, Division of Infectious Diseases, Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine

Combined optical and μCT imaging in a mouse model of orthopaedic implant infection, utilizing a bioluminescent engineered strain of Staphylococcus aureus, provided the capability to noninvasively and longitudinally monitor the dynamics of the bacterial infection, as well as the corresponding inflammatory response and anatomical changes in the bone.